【摘要】 目的 :采用人类 B淋巴细胞株 Raji细胞作为实验模型 ,用东亚钳蝎毒 (BMK)作为干预手段 ,观察其对Raji细胞的生长抑制和凋亡诱导作用 ,并探讨其作用机制。方法 :MTT法 ,Wright- Gimesa染色法 ,Hoechst3334 2和 PI荧光染色 ,透射电镜技术和流式细胞分光光度术。结果 :BMK(12 .5 m g/ L～ 2 0 0 .0 mg/ L)可明显地抑制 Raji细胞生长和诱导细胞凋亡 (P<0 .0 5 ,P<0 .0 1) ,并且其作用强度在一定范围内呈现对浓度和时间的依赖性。流式细胞仪分析显示BMK能下调 Raji细胞 bcl- 2蛋白表达。结论 :BMK能抑制 Raji细胞生长和诱导细胞凋亡 ,其作用机制可能与 bcl- 2蛋白表达的下降有关。更多还原

【Abstract】 Objective:The human B lymphoma cell line Raji were used as an experimental model,BMK as an intervenient means.We investigated the effect and mechanism of BMK on inhibition of cell growth and induction of apoptosis in Raji cell line.Methods:MTT,Wright Gimesa mixed staining,Hoechst 33342 and PI fluorescence staining,ansmission electron microscopic technique and flow cytometry were adopted.Rusults:The results showed that BMK(12.5 mg/L～200.0 mg/L)could inhibit cell growth and induce apoptosis obviously (P<0.05,P<0.01 ),and the effect of BMK was dose and time dependent within a limited range.Flow cytometry showed that BMK could inhibit the expression of bcl 2 protein.Conclusion:BMK could induce apoptosis of Raji cell lin,and the mechanism of effect may relate to the decreased expression of bcl 2 protein.更多还原