【摘要】 目的 :观察三氧化二砷 (As2 O3)诱导 K5 6 2细胞凋亡过程中 Caspase3活性的变化。方法 :K 5 6 2细胞分别用浓度为 0 .5μm ol/ L ,1μmol/ L ,2μm ol/ L ,5μmol/ L的 As2 O3处理不同时间 (0 ,6 h,12 h,2 4h,48h,72 h) ,用流式细胞仪检测 K5 6 2细胞凋亡率 ;荧光发光光度计检测 Caspase3活性。结果 :1μm ol/ L～ 5μm ol/ L的 As2 O3可诱导 K5 6 2细胞凋亡 ,As2 O3浓度在 2μm ol/ L以上时作用更明显。 K5 6 2细胞凋亡过程中 Caspase3活性明显升高。结论 :As2 O3可诱导 K5 6 2细胞凋亡 ,以 2μmol/ L以上的浓度作用更明显 ;Caspase3活化参与了 As2 O3诱导 K5 6 2细胞凋亡的过程更多还原

【Abstract】 Objective:To observation changes of caspase₃ activity in apoptosis of K562 cells induced by arsenic troxide.Methods:K562 cell line were treated with As₂O₃ of 0.5,1,2,5 μmol/L in concentration of 0,6,12,24,48,72 hours respectively.Flow cytometry was used to detect the apoptosis.Spectrofluorometry was used to detect caspase₃ activity.Rusults:K562 cells could be induced to undergo apoptosis by 1 μmol/L～5 μmol/L As₂O₃,especially when the concentration of As₂O₃ was higher than 2 μmol/L.Caspase₃ activity of K562 cells was remarkably increased after the As₂O₃ treatment.Conclusion:The K562 cells can be induced to apoptosis by 1 μmol/L～5 μmol/L As₂O₃,especially when the concentration of As₂O₃ is higher than 2 μmol/L.Caspase₃ activation participates in the processes in inducing the apoptosis of K562 cells.更多还原