【摘要】 胚胎干细胞 ES是从早期胚胎或原始生殖细胞中分离出来的能够在体外进行传代培养 ,而不分化的全能性细胞。ES细胞最主要的生物学特征是具有发育的全能性或多能性 ,ES细胞能在体外培养、增殖、传代、冻存和诱导分化 ,是研究生物发育、行为和建立人体疾病模型的理想工具 ,本文重点论述了影响胚胎干细胞分离和克隆的因素 ,并展望了胚胎干细胞的应用前景更多还原

【Abstract】 Embryo stem cells (ESCs) are a kind of omnipotent cells separated from early embryo or primordial germ cells that can be cultured in vitro for many generations without differentiation. Main biological feature of ESCs is its omnipotence or polypotence and it can be cultivated, multiplied, frozen and induced for differentiation in vitro, which is an ideal tool for researches of animal development, animal behaviors and human illness models. The factors affecting the separation and cloning of embryo stem cell were reviewed and its application prospects in the future were predicted in this paper.更多还原