【摘要】 用荧光法研究了氟罗沙星对牛血清白蛋白的荧光猝灭现象 ,根据荧光猝灭双倒数图计算了氟罗沙星与牛血清白蛋白之间的结合常数 ,根据F ster能量传递原理计算出氟罗沙星在牛血清白蛋白上的结合位置 ,并根据热力学参数确定了氟罗沙星与牛血清白蛋白之间的作用力类型更多还原

【Abstract】 A fluorescence method is used to study the fluorescence quenching of bovine serum albumin by its interaction with fleroxacin. The interaction association constants of bovine serum albumin and fleroxacin are determined from a double reciprocal Lineweaver Burk plot. According to the Fster dipole dipole energy transfer, the distance to be measured between the fleroxacin and tryptophane is 4.37 nm. From thermodynamical coordination it can be judged that the binding power between fleroxacin and bovine serum albumin is static electric power.更多还原