【摘要】 目的 :在人胎儿脑组织 c DNA中钓取并克隆新基因。方法 :以 RT- PCR方法用人 FGF- 19特异性引物从人胎脑 c DNA中扩增到一大小约 6 6 0 bp的片段 ,并连入 TA克隆载体测序 ,得到一个表达序列标签 (EST) ,输入 Gene Bank、Swissprot、db EST等数据库中进行同源比较分析。结果 :分析表明它是人胎儿脑组织未知基因 EST,是一个 6 6 3个碱基可编码 2 2 0个氨基酸的开放读码框架。结论 :在人胎儿脑组织 c DNA中钓取并克隆一个人胎脑新基因 EST。更多还原

【Abstract】 Objective:To obtain and clone the new gene of human fetal brain. Methods: A cDNA fragment about 660 bp was isolated from human fetal brain tissue by RT PCR with human FgF 19 primers. Then it was cloned into pGEM T Easy plasmid to be sequenced. Results: Sequencing analysis proved that it was a novel Expressed Sequence Tag(EST) derived form human fetal brain and contained an open reading frame(ORF) with the size of 663 bp coding for 220aa.Conclusion: A human novel EST was obtained.更多还原