【摘要】 为简便快速诊断急性心肌梗死 (AMI) ,建立一种应用全血定性测定肌红蛋白 (Mb)浓度的免疫层析分析。方法仅需将层析条插入 2 0 0 μL全血与定量层析液的混合液中 1min ,15min内观察结果。如果血Mb浓度≥10 0ng/mL ,15min内即出现阳性红色条带。用全血免疫层析分析和血清Mb -ELISA平行测定 ,94名健康人 ,2 3例AMI患者和 6例心绞痛患者的Mb浓度 ,所得结果完全符合。,对患者血清Mb浓度进行了分析 ,并对Mb测定中应注意的事项进行了讨论更多还原

【Abstract】 In order to diagnose acute myocardial infarction (AMI) ,a simple,rapid, and qualitative immunochromatographic assay of myoglobin(Mb) with whole blood has been established.Just through inserting a chromatographic strip into a mixture of 200μL whole blood and a fixed volume of chromatographic liquid for one minute,then observing the results, if a red line appeared within 15 minutes at the positive line site, serum Mb concentration was considered as positive, indicating Mb≥100ng/mL. Through the qualitative and quantitative detection of serum Mb concentration of 94 healthy persons, 23 cases of AMI patients and 6 cases of cardiac angina patients with Mb immunochromatographic assay and Mb-ELISA in parallel, the results were coincided completely. In analysis of the serum concentration results of AMI and cardiac angina patients, points of attention in serum Mb detection have been mentioned.更多还原