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人工诱导泥鳅与大鳞副泥鳅雄核发育单倍体子代的RAPD分析

RAPD Analysis of Artificially Induced Androgenesis Haploid of Misgurnus anguillicandatus and Paramisgurnus dabryanus

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【作者】 龙良启; 赵振山; 汤保贵; 熊帮喜;

【Author】 LONG Liang\|Qi\ ZHAO Zhen\|Shan\ TANG Bao\|Gui\ XIONG Bang\|Xi (The Agricultural Ministry Key Laboratory of Freshwater Aquaculture and Enhancement Biology in Huazhong Agricultural University\ Wuhan\ 430070,China)

【机构】 华中农业大学农业部淡水水产增养殖生物学重点实验室!武汉430070;

【摘要】 用 30个经过筛选的随机引物对 3组泥鳅雄核发育单倍体、2组大鳞副泥鳅雄核发育单倍体及其相应亲本进行了RAPD分析。结果表明 ,雄核发育单倍体子代与其父本的RAPD谱带相似率为 97 0 %~ 97 8% ,与母本相似率为 30 3%~ 59 5% ,子代中的非亲本谱带率为 0~ 0 0 2 9,极少母本特异谱带。这一结果说明雄核发育鱼类单倍体的遗传信息主要来自父本 ,且存在着个体差异 ,雄核发育子代存在DNA变异和母本DNA非特异带 ,但并非其必要条件。

【Abstract】 An overall RAPD analysis of three groups of adrogenesis haploid of Misgurnus anguillicandatus, two groups of androgenesis haploid of Paramisgunus dabryanus and their parents were made by selected 30 primers of radom decamer oligonucleotide. The results showed:the similarity index was 97\^0%~97\^8% between androgenesis haploid and their male parents; 30\^3%~59\^5% between androgenesis haploid and their female parents ;the non\|parent band ratio of androgenesis haploid was 0~0\^029; mother specific band was rarely found in the androgenetic haploid. It was concluded that the major genetic informations of androgenetic hapolid came from their male parents; moreover there were differences among the androgenetic haploid produced by different sire; the non\|parents bands and maternal specific bands would be unnecessary for androgenesis haploid.

【基金】 国家自然科学基金!资助项目 (No .39770 5 92 )
  • 【文献出处】 动物学杂志 ,Chinese Journal of Zoology , 编辑部邮箱 ,2001年01期
  • 【分类号】Q953
  • 【被引频次】12
  • 【下载频次】127
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