【摘要】 为了探索γ 氨基丁酸 (GABA)对小白鼠离体胃标本胃酸分泌 (GAS)的影响及机制 ,在体外 37℃缓冲液中培育离体、胃腔灌流并维持胃内 12cm水柱压力的全胃标本 ,用 pHS 3型精密酸度计测定灌流液的 pH。结果表明 :γ 氨基丁酸 (GABA) (1～ 10× 10 -7mol/L)和巴氯芬 (Bac,0 6～ 9 6× 10 -7mol/L)以一种浓度依赖的方式显著地促进胃酸分泌 (GAS) ,而西咪替丁 (Cim ,2～ 2 0× 10 -7mol/L)以一种浓度依赖的方式有力地抑制GAS。印防已毒素 (Pic ,3× 10 -7mol/L)不影响基础胃酸分泌 (BGAS)和GABA促进GAS的效应 ,而番氯芬(Phac ,0 .6× 10 -7mol/L)能完全阻断GABA的促进效应。Cim不能完全消除GABA和Bac对GAS的促进效应。以上结果提示 ,在小鼠中GABA可以通过激活胃中GABAB 受体促进离体胃标本的GAS ,可能胃壁胆碱能神经元和非神经细胞 ,如壁细胞及某些内分泌细胞上都存在GABAB 受体 ,GABA可直接或简接地剌激胃壁细胞分泌酸。更多还原

【Abstract】 To explore the effects and the mechanism of γ aminobutyric acid (GABA) on gastric acid secretion (GAS) of isolated mouse stomach with perfused lumen, 12 cm H 2O column intragastric pressure provided. Whole stomach preparations from mice were incubated in buffer at 37℃ in vitro, and perfusate was measured for pH with a pHS 3 type pH meter. The results shown that GABA (1～10×10 -7 mol/L) and baclofen (Bac) (0 6～9 6×10 -7 mol/L) significantly promoted GAS in a concentration dependent manner, whereas cimetidine (Cim) (2～20×10 -7 mol/L) potently inhibited GAS in the same manner. Picrotoxin (Pic) (3×10 -7 mol/L) did not affect basal gastric acid secretion (BGAS) and the promotive effect of GABA on GAS. Phaclofen (Phac) (0 6×10 -7 mol/L) completely inhibited the promotive effect of GABA on GAS. Cim did not completely eliminate the promotive effects of GABA and Bac on GAS in isolated mouse stomach. Above results suggest that in mouse, GABA may promote GAS in isolated stomach, possibly by affecting GABA B receptors on both cholinergic neurons and non neuronal cells, such as parietal cells and some endocrine cells. GABA may stimulate parietal cells to secrete acid directly or indirectly.更多还原