【摘要】 用电穿孔转染法 ,建立稳定表达野生型LMP1及其不同突变体的鼻咽癌细胞系 ,并以这些细胞系为材料 ,用MTT法检测增殖期活细胞 ,观察LMP1及其不同的结构域对鼻咽癌细胞生长的影响。结果得到了LMP1及其三种突变体、空白载体表达的鼻咽癌细胞系 :HNE2 -LMP1(野生型 )、HNE2 -LMP1△ 185 - 35 1(CTAR1缺失型 )、HNE2 -LMP1(1- 2 31) (CTAR2缺失型 )、HNE2 -LMP1(1- 185 ) (羧基端胞浆区缺失型 )、HNE2 -pSG5 (空载体型 )。进一步证实HNE2 -LMP1、HNE2 -LMP1(1- 2 31)、HNE2 -LMP1△ 185 - 35 1平均吸光度 (A )比值高于对照组HNE2 -pSG5及HNE2 (P <0 0 1)。这提示 :EB病毒LMP1及其LMP1(1- 2 31)和LMP1△ 185 - 35 1在体外明显促进HNE2细胞增殖。结果表明EB病毒LMP1可能在鼻咽癌中发挥着重要的作用。更多还原

【Abstract】 The stable transfectant cell lines were established by introducing LMP1 cDNA,LMP1(1-185),LMP1(1-231)(LMP1 CTAR2),LMP1△185-351(LMP1 CTAR1) and the vector (pSG5) into an EBV-negative cell line HNE2 derived from a poorly-differentiated nasopharyngeal carcinoma through electroporation.Using these cell lines as models,the cell proliferation were measured by MTT method.We found the average A ratio in HNE2-LMP1,HNE2-LMP1(1-231) and HNE2-LMP1△185-351 cell lines were higher than those in HNE2-pSG5 and HNE2 cell lines(P<0.05).These data suggest that LMP1,LMP1(1-231) and LMP1△185-351 can obviously promote HNE2 cell growth, EBV LMP1 may play an important role in the development of NPC.更多还原