【摘要】 目的 :探讨结核杆菌感染外周血单个核细胞 (PBMC)对细胞免疫功能的影响及在结核病转归中的作用。方法 :用PCR检测结核病患者PBMC中结核杆菌DNA(TB DNA) ,用生物素 链霉亲和素 (BSA)系统同步检测其T细胞亚群及经植物血凝素 (PHA)诱导前后膜白介素 2受体 (mIL 2R)水平。结果 :结核病患者T细胞亚群及mIL 2R水平与对照组相比均显著降低(P <0 .0 5～P <0 .0 1)。其中PBMC内TB DNA(+)组与TB DNA(- )组相比 ,CD3+ 、CD4+ 百分率及CD4+ /CD8+ 比值降低 ,CD8+ 百分率增高 (P <0 .0 5 ) ;PHA诱导前后mIL 2R水平较对照组相比均低下 ,差异均有显著性 (P <0 .0 1)。结论 :结核病患者体内细胞免疫功能低下 ,结核杆菌感染PBMC后可加重患者细胞免疫功能紊乱 ,抑制其mIL 2R表达。更多还原

【Abstract】 Objective:To investigate the influence of Mycobacterium tuberculosis (Mtb) infection in peripheral blood mononuclear cells(PBMC) on the function of cellular immunity and its effects on the transformation of tuberculosis.Methods:The Mtb DNA in PBMC was detected by polymerase chain reaction(PCR),and phenotypes of T cell subsets and the expressing level of membrane interleukin 2 receptor(mIL 2R) with or without PHA inducement were detected by biotin streptavidin(BSA) technique.Results:Both the proportion of T cell subsets and the level of mIL 2R were decreased in patients with tuberculosis than those in normal controls( P <0.05～ P <0.01).While the proportion of CD3 + and CD4 + cells in PBMC,the ratio of CD4 +/CD8 + cells,and the level of mIL 2R in PBMC were significantly lower in Mtb DNA(+) group than those in Mtb DNA(-) group( P <0.01),the proportion of CD8 + cells in PBMC was higher in Mtb DNA(+) group than that in Mtb DNA(-) group( P <0.05).Conclusions:The results in this study showed that the cellular immunity was obviously lower in patients with tuberculosis.The disorder of cellular immunity in patients with tuberculosis was further aggravated and the level of mIL 2R was restrained by infection of Mtb in PBMC.更多还原