【摘要】 建立了一种用XterraRP1 8色谱柱快速分离测定水解氨基酸的方法。所采用的色谱条件是 :WatersAlliance系统 ,柱温 5 6℃ ,流速 1 .8ml/min ,检测波长 2 4 8nm ,梯度分离 ,运行周期 2 5min,柱反压低于 2 0 0 0Psi。在 1 7.5min内分离了包括AMQ、NH3 和牛磺酸在内的 2 1种氨基化合物 ,适应于复合氨基酸注射液、含牛磺酸的氨基酸口服液及水解氨基酸样品的分析测定更多还原

【Abstract】 A method for rapid separation and determination of amino acids by high performance liquid chromatography on a Xterra RP18 column was established. The chromatogram conditions were: the column temperature of 56℃, the flow rate of 1.8 ml/min and the detecting wavelength of 248nm; gradient separation on the Waters Alliance TM system, the run time of 25 minutes and the counterpressure of chromatogram column under 2000 psi. 21 amide was separated within 17.5 minutes, and AMQ, NH 3 and taurine were included. The method could be used for the determination of amino acids in compound amino acid injection liquid, oral amino acid liquid including taurine and compound amino acid hydrolyzed sample.更多还原