【摘要】 目的:了解肺癌患者肺泡巨噬细胞(alveolarmacrophage,AM)产生细胞因子的情况及其细胞因子的细胞杀伤活性,同时观察γ干扰素(γinterferon,γ-INF)和脂多糖(lipoplysaccharide,LPS)对AM活性的影响。方法:经肺泡灌洗获AM,分离,γ-INF和/或LPS刺激培养,分别检测AM产生NO、IL-1和TNF-α的量以及其细胞杀伤活性。结果:肺癌患者的部分AM,即使未经刺激,亦分泌细胞因子并具有细胞杀伤活性;经γ-INF和LPS刺激后,两者均使AM分泌细胞因子增多和增强其细胞杀伤活性,且联合刺激较单独刺激对AM的作用影响大。结论:肺癌患者临近肿瘤组织的部分AM活性增强;γ-INF和LPS能刺激AM产生细胞因子并提高其细胞杀伤活性,且两者具有协同作用。更多还原

【Abstract】 Objective:The aim of this study was to observe the function that the alveolar macrophages(AM) produce cell factors and kill cells from the patients with lung cancer, and to observe the effect that γ INF and LPS stimulate the AMs. Methods: The AMs were collected by bronchoalveolar lavage, divided and cultured by stimulated with γ INF and/or LPS. The function that the AMs produce cell factors and kill cells was examined. Results: A part of AMs from the patients with lung cancer have been activated. The function that the AMs produce cell factors and kill cells was enhanced after stimulated with γ INF and/or LPS. The function was best after stimulated with γ INF and LPS. Conclusion: The activity of the AMs close to the tumor from the patients with lung cancer was enhanced. γ INF and LPS can enhance effect of AMs producing cell factors and killing cells with best effect after stimulated with combination of γ INF and LPS. and that is best after stimulated with γ INF гand LPS.更多还原