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牡丹组织培养技术研究

Study on tissue culture techniques for peony

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【作者】 张桂花王洪梅王连祥

【Author】 ZHANG Gui-hua, WANG Hong-mei, WANG Lian-xiang (Heze Institute of Agricultural Sciences, Heze 274000, China)

【机构】 菏泽市农科所高新技术研究室菏泽市农科所高新技术研究室 山东菏泽274000山东菏泽274000山东菏泽274000

【摘要】 于牡丹芽萌发期和休眠期分别进行腋芽和顶芽组织培养。结果表明 ,自芽萌动至开始休眠为适宜培养期。芽诱导最佳培养基为 :MS +6 -BA(0 5~ 1mg/L) +NAA(0 1~ 0 2mg/L) ,MS为最佳壮苗培养基。牡丹试管苗培养的适宜光照强度为 2 0 0 0lx ,光照时数为 10h/天 ,适宜温度 (2 5± 1)℃。

【Abstract】 The axillary bud and terminal bud of peony were cultured at germinating stage and dormant stage The result showed that the suitable culture stage was from bud germinating to dormant beginning The optimum medium for inducing bud was MS+6-BA(0 5~1mg/L)+NAA(0 1~0 2mg/L),and MS was the optimum medium for healthy seedling For tube seedling, the suitable light intensity, time and temperature were 2000lx, 10h/d and (25±1)℃ respectively

【关键词】 组织培养牡丹培养基
【Key words】 Peonytissue culturemedium
  • 【文献出处】 山东农业科学 ,Shandong Agricultural Sciences , 编辑部邮箱 ,2001年05期
  • 【分类号】S685.11
  • 【被引频次】107
  • 【下载频次】751
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