【摘要】 目的 :观察轻度低温对脑缺血再灌注后细胞凋亡的影响。方法 :沙鼠 4 0只 ,随机分假手术组、缺血组和轻度低温 ( 3 2～ 3 3℃ ) 2 h组、6h组、1 2 h组共 5组。夹闭双侧颈总动脉 2 0 min再灌注 72 h观察细胞凋亡。结果 :FCM显示缺血组海马区凋亡细胞 9.3 % ,轻度低温 2 h、6h、1 2 h组分别 4 .6%、2 .6%、1 .7%。 HE显示缺血组海马CA1 区损伤细胞为 2 6.4 % ,低温 1 2 h下降为 4 .4 %。结论 :轻度低温能抑制脑缺血后凋亡及坏死细胞。更多还原

【Abstract】 Objective:To observe the protective effects of mild hypothermia on the apoptosis of hippocampal neurons after cerebral ischemia and reperfusion injury.Methods:40 gerbils were divided into five groups:sham～operated,ischemia and mild hypothermia 2h,6h and 12h,respectively bilateral common carotid arteries and were occluded for 20 min and reperfusion for 72h,apoptosis was determined by FCM and HE dye.Results:The percentage of apoptotic cells was 9.7% in ischemia group,mild hypothermia 2h,6h,12h,could reduced the apoptotic cells (4.6%,2.6%,1.7%) respectively.The number of abnormal neurons (26.4%). was higher in ischemia group In contrast,the number of abnormal neurons were reduced lower (14.8%,8.3%,4.4%) in the mild hypothermia (2h,6h,12h).Conclusion:Mild hypothermia Could reduced apoptosis and necrosis following cerebral ischemia and reperfusion injury.更多还原