【摘要】 目的 :观察补肾中药治疗前后雄激素致高胰岛素高雄激素不孕大鼠胰岛素样生长因子 1(IGF 1)及胰岛素样生长因子 1受体 (IGF 1R)的变化。方法 :建立雄激素致高胰岛素高雄激素不孕大鼠模型 ,通过阴道涂片筛选各组大鼠 ;血清IGF 1测定采用放免法 ,IGF 1R的检测采用免疫组化法。结果 :( 1)血清IGF 1水平 :模型组 >模型服中药组 >正常组 ,但各组间比较差异无显著性。 ( 2 )胰腺、肝脏、卵巢和肾上腺IGF 1R免疫组化定量分析 :模型组 <模型服中药组 <正常组。结论 :雄激素致不孕大鼠存在着高水平血清IGF 1及主要靶器官低水平IGF 1R ,而补肾中药对其有一定的调节作用。由此推测 ,补肾中药对雄激素致不孕大鼠的促排卵作用可能是部分通过调节IGF 1/IGF 1R来实现的更多还原

【Abstract】 To observe the effect of Kidney replenishing Chinese herbs (KRCH) on insulin-like growth factor-1 and its receptor (IGF-1/IGF-1R) in androgen induced sterile rats (ASR). Methods: ASR model was set up and screened by vaginal smear. The level of serum IGF-1 was detected by radioimmunoassay, and IGF-1R by immunohistochemical method. Results: (1) Serum IGF-1 level in model rats group > model rats treated with KRCH group > normal control group, but no statistical difference was found among them. KRCH could lower the level of serum IGF-1. (2) Level of IGF-1R in pancreas, liver, ovary and adrenal gland showed an opposite sequence to that of serum IGF-1. Conclusion: ASR shows a high IGF-1 level in serum and low IGF-1R level in main target organs, and KRCH could adjust the levels in certain degree. From this, it is inferred that the ovulation effect of KRCH on ASR may come true partly by adjusting the level of IGF-1/IGF-1R.更多还原