节点文献
救脑宁注射液对谷氨酸、5-羟色胺诱发大鼠皮质神经细胞内游离钙超负荷的影响
Effect of Jiunaoning Iujection on Overload of Intracellular Free Calcium of Cerebral Cortex Induced by Glutamic Acid or 5-Hydroxytryptamine in Fetal Rats
【摘要】 目的:探讨救脑宁注射液对谷氨酸(Glu)、5-羟色胺(5-HT)诱发大鼠皮质神经细胞内游离钙(〔Ca2+〕i)超负荷的影响。方法:以Fura-2/AM为细胞内钙离子的荧光指示剂,用双波长荧光分光光度计测定了急性分离的新生大鼠神经细胞〔Ca2+〕i的变化,观察Glu、5-HT对〔Ca2+〕i的影响及救脑宁注射液的干预作用。结果:Glu和5-HT剂量依赖性引起〔Ca2+〕i增加,导致细胞内钙超载;救脑宁注射液可显著降低细胞内钙的升高,保护神经细胞免受Glu和5-HT的损害。结论:救脑宁注射液对中风病的治疗作用与其对抗Glu、5-HT等毒性物质的作用,抑制受体依赖性钙通道的开放,减少外钙的流入,有效地防止钙超载等机制有关。
【Abstract】 Objective: To explore the effect of Jiunaoning Injection (JNNI) on overload of intracellular free calcium of cerebral cortex induced by glutamic acid or 5-hydroxytryptamine (5-HT) in fetal rats. Methods: Double wave length spectrofluorometer with Fura-2/AM as the fluorescence indicator for intracellular calcium ions (〔Ca2+〕i) was used to measure the changes of 〔Ca2+〕i in instantly separated cortical nerve cells of newborn rats affected by glutamic acid or 5-HT, as well as the interference of JNNI on it. Results: Glutamic acid or 5-HT could elevate the intracellular 〔Ca2+〕i dose-dependently and induce 〔Ca2+〕i overload. JNNI could restrain the elevation markedly so as to protect the neurocytes from injury of glutamic acid and 5-HT. Conclusion: The therapeutic ef fect of JNNI in treating apoplexy is related with its action in suppressing the toxic substances as glutamic acid and 5-HT, restraining the opening of receptor dependent calcium channel, reducing the external cellular calcium influx and preventing the calcium overload effectively.
【Key words】 Jiunaoning Injection; intracellular free calcium; Fura-2/AM; nerve cell;
- 【文献出处】 中国中西医结合杂志 ,CHINESE JOURNAL OF INTEGRATED TRADITIONAL AND WESTERN MEDICINE , 编辑部邮箱 ,2000年06期
- 【分类号】R285
- 【被引频次】6
- 【下载频次】62