【摘要】 目的　研究羟基喜树碱体外诱导人肺癌细胞株SPC-A-1 凋亡的作用。方法　以不同浓度的羟基喜树碱处理在体外培养的肺癌细胞,以电子显微镜、流式细胞仪和原位末端标记分析来检测细胞凋亡。结果　在0.05 m g/m l浓度下,羟基喜树碱作用于体外培养的肺癌细胞20 h 后,肿瘤细胞的凋亡率在8.7 % ～10.4 % 之间,主要以早、中期的表现为主;在0.1 m g/m l浓度下,作用20 h 后,凋亡率为18.6% ,凋亡形态学表现主要以晚期为主。结论　羟基喜树碱可作为细胞凋亡诱导剂用于肺癌治疗更多还原

【Abstract】 Objective To study the effect of hydroxyl camptothecinum on human lung cancer cell apoptosis in vitro.Methods SPC A 1 cell line was treated with hydroxyl camptothecinum at different concentrations.Cell apoptosis was evaluated by electron microscopy,flow cytometry,and in situ TUNEL (TdT mediated dUTP X nick end labeling).Results The apoptosis rate of lung cancer cell treated with hydroxyl camptothecinum at the concentration of 0.05 mg/ml for 20 hours was between 8.7 % 10.4 %,mainly at early and middle apoptosis stage.At the concentration of 0.1 mg/ml,the rate was 18.6 %,mainly at late stage.Conclusion Hydroxyl camptothecinum is a potential agent for lung cancer chemotherapy.$$$$更多还原