【摘要】 目的　建立酶联免疫吸附实验 (EL ISA)测定苯妥英钠血药浓度的方法。方法　采用碳二亚胺法将化学修饰后的苯妥英钠与人血清白蛋白结合 ,再与被氧化的辣根过氧化物酶结合 ,作为酶标抗原 ,自制兔抗苯妥英钠多克隆抗体。结果　抗苯妥英钠 Ig G稀释倍数 1∶ 2 0 0、酶标记抗原 1∶ 10 0时 ,标准曲线最理想 ;该方法的测定灵敏度为 2 .87μg/ ml,线性范围 2 .9～ 30 μg/ m l,批内变异系数 3.3%～ 10 .2 % ,批间变异系数 5 .1%～ 13.2 % ,回收率 90 %～ 96 % ,其它 4种抗癫痫药物 (扑痫酮、苯巴比妥、卡马西平和丙戊酸钠 )对测定无干扰 ,与 HPL C方法相关良好 ,相关系数为 0 .97。结论　该方法具有良好的准确性、精确性和特异性 ,灵敏度和测定范围能够满足临床需要 ,可用于苯妥英钠的临床治疗药物监测。更多还原

【Abstract】 Objective To develop a solid phase,competitive enzyme immunoassay for the measurement of serum phenytoin. Methods The chemical modified phenytoin combined with human serum albumin and then conjugated with horseradish peroxidase (HRP) to produce the enzyme labeled phenytoin(DPH HSA HRP).The anti phenytoin antibody was prepared in this lab. Results The working range and sensitivity of this method were 2 9~30 μg/ml and 2 87 μg/ml respectively.The intro assay coefficient of variation (CV) was 3 3%~10 2% ( n=15 ) and inter assay CV was 5 1%~13 2%.The recovery of this method was 90%~96%.N