【摘要】 从贯叶金丝桃叶和嫩茎诱导愈伤组织的过程,探讨了悬浮培养和平板培养的方法,以及细胞悬浮培养时间、培养方法和接种密集度对贯叶金丝桃单细胞平板培养植板率的影响。在所实验的MS、B₅、White₃种培养基中,以MS培养基较好,其中MS+2,4-Dlmg/L+NAA1mg/L培养基愈伤组织诱导率为92.86％。细胞悬浮培养以B₅培养基较好。普通单细胞平板培养法培养贯叶金丝桃单细胞的植板率很低,而以悬浮培养14～21d的单细胞为材料,接种密度5×10³/ml时,进行条件培养和看护培养,植板率达11.62％。更多还原

【Abstract】 Callus were induced from the leaves and stems of Hypericum perforatun L. The cell suspension culture and plate culture were also investigated in this paper.The results showed that the medium of MS which contained 2,4-D Img/L and NAA Img/L was the best one for inducing callus.The plant growth regulator of 2,4-D was assential for callus and suspension culture. Factors such as the time of cell suspension culture, the ways of plating and cell density, which affects the plating efficiency were studied.The results showed that plating at density of 5×103 cells/ml and with monocells seperated from the cells cultured 14 -21 days,high plating efficiency could be obtained either by conditional plate culture or by nursing plate culture.更多还原