【摘要】 利用差示技术从红豆杉悬浮细胞中克隆出茉莉酸类诱导表达的基因 ,并用异源表达系统对有关基因进行功能鉴定 ,从而阐明紫杉醇生物合成机制 ,为今后解决红豆杉悬浮法生产细胞紫杉醇产量低且不稳定的难题提供理论依据和技术手段。更多还原

【Abstract】 In order to understand the mechanism of taxol biosynthesis, a new idea of using Jasamonates and mRNA differential display to clone the jasamonates elicited gene in taxus suspension cells and their functional analysis with heterologous expression system is proposed. This strategy may provided a solution to the problem of low and unstable taxol yield in taxus suspension cells.更多还原