【摘要】 利用酸化处理的Sepharose 6B亲和柱从洋紫荆种子中分离纯化出了洋紫荆凝集素 (BVL) ,其比活性比抽提液提高了 1 59倍 ,活力回收率为 49.0 %。BVL分子量为 81 0 0 0 ,由两个相同的亚基组成。等电聚焦凝胶电泳测得其等电点为 4.95。其紫外吸收高峰在 2 76nm处。BVL具一定的热稳定性和酸碱稳定性。N 乙酰 D 氨基半乳糖能强烈地抑制BVL对兔红细胞的凝集作用 ,乳糖、半乳糖也有较强的抑制作用。更多还原

【Abstract】 Bauhinia variegata lectin(BVL) has been purified from the seed of Bauhinia variegata L. by affinity c hromatography on an acid-treated Sepharose 6B column . The hemagglutinating act ivity of the purified BVL increased 159 times and the activity recovery is 49.0 %. The molecular weight is 81 000 and the molecule consists of two identical sub units. Its isoelectric point is 4.95. N-acetyl-D-galactosamine is the most p otent inhibitors of BVL. Lactose and galactose can also inhibit the agglutinati on of rabbit erythocytes by BVL.更多还原