【摘要】 研究人脐静脉内皮细胞(HUVEC)和大鼠脑微血管内皮细胞(RCEC)的有丝分裂过程及其运动特征。方法用TimeLapseRecorder8050缩时录像机将人脐静脉内皮细胞及大鼠脑微血管内皮细胞活动的状态进行昼夜36h连续记录 ,然后以正常速度回放磁带 ,经MCIP图像处理系统进行图像分析 ,使细胞的有丝分裂过程在加速24倍的短时间内得到更加清晰的动态再现。结果在细胞分裂间期 ,HUVEC处于动态变化过程中 ,细胞膜以“波浪式”不停地运动 ,并不断地向四周伸出扇形伪足。而RCEC则是显现“阿米巴”样的运动并以泡状伪足向四周不断延伸。HUVEC整个有丝分裂过程所需时间为(31.5±4.4)min(n=15),而RCEC有丝分裂全过程则需(29.7±3.0)min(n=12)。结论HUVEC和RCEC有丝分裂过程所需时间没有明显区别 ,但两种细胞的运动特征却不相同 ,其机理有待深入研究更多还原

【Abstract】 Objective In order to explore the influence of vasoactive substances on the proliferation of endothelial cells. Methods The mitotic dynamics of cultured endothelial cells from human umbilical cord vein(HUVEC)and rat cerebral microvessels were observed via phase_contrast microscope and time_lapse videomicroscopy for 36 hours continuously, and was reviewed at a play_back speed of 24 times normal. The dynamic features and the mitotic duration of these cells were recorded via long time slow recorder. Then the recorded cell activities were analyzed by image analysis system(MCIP). The duration for mitotic process of each selected endothelial cell was counted according to the digital timer generated on screen during recording .Results The result showed that the dynamic features of HUVEC and RCEC were different. The HUVEC exhibited undulating and streaming motions with successively extending 2～3 fanlike lamellipodias simultaneously. But the RCEC exhibited active motions with extending 5～6 vesiclelike podias simultaneously. The mitosis duration for HUVEC was 31.5±4.4 minutes(n=15),but for RCEC was 29.7±3.0 minutes (n=12).Conclusions The information provided in this study was not available previously. These findings opened a new aspect for further investigations to the effect of pharmaceutical and pathophysiological factors influencing on function of human being and animals.更多还原