【摘要】 应用末端脱氧核苷酸转移酶介导的dUTP原位切口末端标记 (TUNEL)检测技术和显微图像定量分析方法研究了悬浮培养中南方红豆杉 (Taxuschinensisvar.mairei)细胞团不同区域细胞凋亡的发生 ,并比较了不同区域的细胞形态。结果显示细胞团可以划分为三个不同的区域 :核心区的细胞结构已消失 ,可见一些分化良好的管状结构 ;中层区是由增殖能力旺盛的分生组织细胞所构成 ;外层区是由分化成熟的细胞所组成。细胞凋亡主要发生在细胞团分化成熟的外层区。以上结果说明细胞凋亡可能是分化终结的一种形式更多还原

【Abstract】 Apoptosis and morphology of different region cells in Taxus chinensis var. mairei cell aggregates in suspension culture were studied with TUNEL method and microspectrophotometric analysis. The results showed that the cell aggregates could be divided into three regions: the central, the middle and the outer region (Plate I a). Cellular structure disappeared except for vessels in the central region. The middle region was composed of meristematic tissue whose cells had high proliferative activity (Plate I b). The cells were better differentiated in the outer region,where apoptosis occurred (Plate Ⅰ c,d). The study suggests a close relation between apoptosis and differentiation. Apoptosis could be an end of differentiation.更多还原