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甘露寡糖和粪链球菌对鸡细胞免疫和肠道微生态的调节作用

Effects of Mannan-oligosaccharide and Streptococcus Faecium Supplemented in Diet on Cell-mediated Immunity and Intestinal Microflora and pH in Chickens

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【作者】 邵良平周伦江李国平林藩平

【Author】 SHAO Liang ping 1, ZHOU Lun jiang 2, LI Guo ping 1, LIN Fan ping 1 (1.College of Animal Science, Fujian Agricultural University, Fuzhou 350002, China; 2. Fujian Institute of Animal Husbandry and Veterinary, Fuzhou 350013, China)

【机构】 福建农业大学动物科学学院!福建福州350002福建省农业科学院畜牧兽医研究所!福建福州350013

【摘要】 报道了日粮中添加甘露寡糖 ( MOS)、粪链球菌 ( SF- 6 8)对鸡细胞免疫和肠道微生态的调节作用 ,以及对血液 SOD和 GSH- Px活力的影响。6 0只 1日龄海兰褐色蛋鸡雄性雏鸡随机分成 4组 :试验 组 (每千克日粮中添加 2 g MOS)、试验 组 (每千克日粮中添加 6 0 mg SF- 6 8)、试验 组 (每千克日粮中添加 2 g MOS和6 0 mg SF- 6 8)和对照组。试验期 4 2 d。结果如下 :( 1) PHA淋巴细胞转化率 ( %) ,试验 组 ( 47.1± 3.0 71)、试验 组 ( 46 .7± 1.76 7)、试验 组 ( 48.3± 2 .312 )都极显著高于对照组 ( 43.8± 1.814 ) ( P <0 .0 1) ;( 2 )白细胞的吞噬率 ( %) ,试验 组 ( 2 5.3± 1.94 7)、试验 组 ( 2 5.3± 2 .54 1)都显著高于对照组 ( 2 3.6± 1.6 47) ,试验 组 ( 2 7.2± 1.889)极显著高于对照组 ( P <0 .0 1) ,同时显著高于试验 、 组 ( P <0 .0 5) ;( 3)盲肠和回肠内容物的 p H值 ,试验 组 ( 6 .2 53± 0 .2 94 ,5.2 58± 0 .4 53)、试验 组 ( 6 .0 2 1± 0 .4 18,5.132± 0 .37)、试验 组 ( 6 .12 4± 0 .2 4 1,5.0 11± 0 .111)都极显著低于对照组 ( 6 .6 6 7± 0 .2 12 ,6 .0 8± 0 .4 12 ) ( P <0 .0 1) ;( 4)每克盲肠内容物大肠杆菌数 (亿 ) ,试验 组 ( 9.16 4± 0 .16 7)显著低于对

【Abstract】 The effects of mannanoligosaccharide (MOS) and streptococcus faecium cernelle 68 (SF 68) in diet for chicken on cell mediated immunity, intestinal parameters and SOD, GSH Px in blood were reported. 60 one day old male chickens of Hy line were divided randomly into 4 groups: group one (basic diet containing 0.2% MOS), group two (basic diet containing 60×10 -6 SF 68), group three (basic diet containing 0.2% MOS and 60×10 -6 SF 68) and control (basic diet). All chickens were slaughtered in 42 days old. The results showed that (1) PHA lymphoblast transformation rate in group one (47 1±3 071), group two (46 7±1 767) and group three (48 3±2 312) is great significantly higher than that in control (43 8±1 814)(P<0 01);(2) macrophage activity in group one (25 3±1 947) and group two (25 3±2 541) is significantly higher than that in control (23 6±1 647)(P<0 05); macrophage activity in group three (27.7±1.889) is significantly higher than that in control (P<0.05), and significantly higher than that in both group one and two (P<0 01); (3) pH in ceca and ileum of group one (6 253±0 294,5 258±0 453), group two (6 021±0 418,5 132±0 37) and group three (6 124±0 241,5 011±0 111) is greatly significantly lower than that of control (6 667±0 212,6 08±0 412)(P<0 01);(4) E.coli in ceca of group one (9 164×10 8) is great significantly lower than that of control (9 412×10 8)(P<0 05);E.coli in ceca of group two (8 748×10 8) and group three (8 726×10 8) are great significantly lower than that of control (P<0 01) and group one (P<0 01); (5) SOD and GSH Px in blood of group one (9 446±0 56,7 541 6±370 01), and group two (9 42±1 005,7 412.1±482 08) are significantly higher than those of control (8 446±0 292,7 064 7±391 03) (P<0 01),SOD and GSH Px in blood of group three (9 789±1 012,7 655 5±266 17) are great significantly higher than those of control (P<0 01). It can be concluded that cell mediated immunity, SOD, GSH Px in blood are stimulated or enhanced and E.coli counts of ceca and pH of ceca and ileum are reduced by improvement of intestinal microflora of chickens with MOS and SF 68 treatment in separation or combination. But macrophage activity and SOD, GSH Px in blood are great effectively higher in the combination of MOS and SF 68 than in other treatments.

【关键词】 甘露寡糖粪链球菌SODGSH-Px益生素
【Key words】 immunostimulationSODGSH Pxintestinal parameterchickenprobiotic
【基金】 福建省自然科学基金!(C960 2 5)
  • 【文献出处】 中国兽医学报 ,CHINESE JOURNAL OF VETERINARY SCIENCE , 编辑部邮箱 ,2000年01期
  • 【分类号】S852.6
  • 【被引频次】68
  • 【下载频次】406
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