【摘要】 目的　了解非灰髓炎肠道病毒 (EV)感染在婴幼儿急性下呼吸道感染 (ALRI)中的发病情况、临床特点及诊断方法。方法　①采用通用引物RT -PCR及病毒分离检测全年住院ALRI婴幼儿鼻咽分泌物 (NPS)中的EV。②并用APAAP法检测RSV、IFV、PIV和AdV ,以资比较。结果　①观察组 87例NPS中RT -PCR阳性 40例 ,EV分离阳性 2 6例。②阳性病例中同时检出其它呼吸道病毒 2 6例。结论　①近半数住院ALRI婴幼儿存在EV感染 ,且常与其它呼吸道病毒混合感染。②EV -RT -PCR较EV分离敏感、实用。③EV感染无临床特异性更多还原

【Abstract】 Objective To investigate the incidence,clinical features and diagnostic methods of non-polio enterovirus(EV) infection in acute lower respiratory infection in infants and young children.Methods ①EV from nasopharygeal secretions(NPS) was detected by RT-PCR and virus isolation in 87 in-patients with ALRI.②RSV,IFV,PIV and ADV were detected by enzyme immunohistochemistry assay.Results ①40 out of 87 patients had EV infection in RT-PCR and 26 in EV isolation.②26 patients were found to be coinfected with other respiratory viruses.Conclusion ①nearly half of the patients with ALRI had EV infection and often coinfected with the other respiratory viruses.②EV-RT-PCR is more sensitive and practical than EV isolation.③EV infection has no clinical specificity.更多还原