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不同血清学方法对调查现场包虫病人血清的检测及评价

THE EXAMINATION AND EVALUATION ON SERA OF 206 CASES WITH HYDATID DISEASE FOUND IN SURVEY SCENE BY DIFFERENT SEROLOGICAL METHODS

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【作者】 王虎曹得萍马淑梅江莉柴君杰

【Author】 WANG Hu,CAO Deping,MA Shumei,JIANG Li,CHAI Junjie (The institute for parasitic disease,Qinghai Medical College Xining 810001)

【机构】 青海医学院寄生虫病研究所!西宁810001卫生部包虫病防治培训基地

【摘要】 本文应用青海囊型包虫抗原 Dot- EL ISA、IHA和新疆囊型包虫抗原 EL ISA、泡型包虫 EM18抗原 EL IB对流行病学调查现场的 2 0 6例包虫病人血清进行了检测。结果表明青海囊型包虫抗原 Dot- EL ISA和 IHA对囊型包虫病人血清的阳性率分别是 90 .37%和 91.98% ;新疆囊型包虫抗原 EL ISA对囊型包虫病人血清的阳性率为 75 .94% ;三种方法对钙化灶型囊型包虫病人血清的阳性率分别是 77.2 7%、81.82 %和 6 5 .91% ,显著低于其它囊型包虫病人血清的阳性率 ;阴性者主要是单纯性肺脏和肝脏囊型包虫病人血清。3种方法 B超、X线诊断的泡型包虫病人的血清阳性率均为 10 0 .0 %。泡型包虫抗原 EM18-EL IB检测结果表明 ,B超 ,X线诊断的泡型包虫病人血清阳性率为 73.6 8% ;囊型包虫病人血清阳性率为 5 .88% ,其中钙化灶型病人血清阳性率为 15 .91% ;阳性血清数与阴性血清数比例约为 1:7(2 5 :181)。对不同方法在囊型和泡型包虫病诊断与鉴别诊断中的价值及意义进行了讨论

【Abstract】 The sera of 206 cases with hydatid disease diagnosed by B-ultrasound and X-ray in survey scene had been examined by Dot-ELISA and IHA with Qinghai cystic hydatid antigen,ELISA With Xingjiang cystic hydatid antigen and Em18-EliB with alveolar hydatid antigen.The results showed that the sero-positive rates were 9037% and 91.98% in these cases with cystic hydatid disease by Dot EliSA and IHA with Qinghai cystic hydatid antigen respectively.The sero-positive rate was 75.94% in same cases by ELISA with Xingjiang cystic hydatid antigen.The sero-positive rates were 77.27% 81.82% and 65.91% in those cases with the whole calcific cystic hydatid disease by above three methods respectively,and the sero-positive rates were lower in whole calcific cystic hydatid than that in other cystic hydatid disease.The sero-negative cases belonged to cystic hydatid disease which located in lungs of livers alone.The results by EM18-ELIB with alveolar hydatid antigen showed that the sero-positive rates were 73.68% and 5.88% in those cases with alveolar hydatid disease and with cystic hydatid disease diagnosed by B-ultrasound and X-ray respectively,and the sero-positive rate was 15.91% in whole calcific cystic hydatid disease.The ratio of the number of positive seras to that of negative seras was 1 to 7 approximately.The value and mean of different serological methods in diagnosis and judge diagnosis for cystic and alveolar hydatid disease had been discussed.

【基金】 国家自然科学基金重点项目!(No;39730 40 0 )
  • 【文献出处】 中国人兽共患病杂志 ,CHINESE JOURNAL OF ZOONOSES , 编辑部邮箱 ,2000年06期
  • 【分类号】R532.32
  • 【被引频次】14
  • 【下载频次】103
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