【摘要】 目的　了解重组的日本血吸虫中国大陆株脂肪酸结合蛋白 (r Sj- FABPc)的特性及预测其抗原表位。方法　以PCR方法从 Sj- c DNA文库中扩增 Sj- FABPc基因片段 ,并亚克隆于载体 p GEM- T,对其核苷酸序列进行测定。以 GOL DKEY○R软件和 Swiss- Model数据库分析 Sj- FABPc的核苷酸序列和其编码的蛋白质特性 ,预测重组抗原的表位。结果　核酸序列分析证明 ,克隆基因确为 Sj- FABPc,并由此推导出 Sj- FABPc理论氨基酸顺序 ,以该序列的分析表明 ,重组 Sj- FABPc抗原具有 3个抗原表位区和三个可能的脂肪酸结合表位。结论　 Sj- FABPc抗原表位可能与脂肪酸结合表位的主要片段为同一肽段。更多还原

【Abstract】 Aim\ To analyse the characteristic and antigen epitope of recombinant antigen Sj-FABPc,Methods\ The cloned gene of FABP was amplified by PCR from a Schistosoma japonicum adult worm cDNA library and subcloned into plasmid pGEM-T.The sequence was analysed by GOLDKEY DNA and Protein Analytical Program and Swiss-Model Protein Modelling server in Internet.Results\ The Tesult indicated that the cloned gene was Sj-FABPc.Three possible antigenic epitopes and three sites of binding fatty acid,of recombinant antigen ofSj-FABPc,were predicted by computer.Conclusions\ The antigen epitopes of Sj-FABPc and the main sites of binding fatty acid may share same peptide.更多还原