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福建棘隙吸虫广东株与福建株的LDH同工酶及蛋白质分析
ANALYSES ON LDH ISOENZYME AND PROTEIN FROM BOTH GUANGDONG STRAIN AND FUJIAN STRAIN OF ECHINOCHASMUS FUJIANENSIS
【摘要】 目的 探讨福建与广东两地福建棘隙吸虫生化特点。方法 用不连续聚丙烯酰胺凝胶电泳及薄层等电聚焦电泳等进行比较分析虫体乳酸脱氢酶 (LDH)同工酶和蛋白质。结果 福建与广东两地虫株都有 5种LDH同工酶 ,其中LDH1、LDH4 和LDH5的迁移率相同 ;蛋白质电泳都分离出 9条区带 ,其中有 7条带的等电点相同。但在LDH2 和LDH3 的迁移率 ,蛋白质区带的相对含量 ,同工酶的总活力与比活力 ,以及A和B亚基的含量等存在差异。结论 LDH同工酶与蛋白质的某些差异 ,可归因为基因型相似条件下所出现两地虫株生化遗传上差异
【Abstract】 Aim\ Inguired into the biochemical characteristrics of Echinochasmus fujianensis found in Fujian and Guangdong provinces. Methods\ Compared and analysed two strains by LDH isoenzyme eletrophoresis, discontinuous PAGE and thinlayer isoelectric equilibrium PAGE.Results\ Two strains both had five LDH isoenzyme, among them, LDH\-1、LDH\-4 and LDH\-5 had the same mobilities. There were nine bands in protein electrophoresis. Seven bands had the same isoelectric point.But there were differences in the mobilities of LDH\-2 and LDH\-3,the comparative amount of protein bands, total and specific activity of isoenzyme and the amount of A and B subunit. Conclusion Under the similar condition of genotypes, the differences of the biochemical inheritance can be contributed to the differences of LDH isoenzyme and protein.
【Key words】 Echinochasmus fujianensis; Strains; LDH isoenzyme; Protein;
- 【文献出处】 中国人兽共患病杂志 ,CHINESE JOURNAL OF ZOONOSES , 编辑部邮箱 ,2000年05期
- 【分类号】R383.2
- 【被引频次】2
- 【下载频次】37