【摘要】 使用PCR技术对 19份福建省HIV - 1阳性感染者或病人的外周血淋巴细胞 (PBMCs)进行扩增 ,获得HIV - 1膜蛋白 (env)基因的部分核酸片段 ,并对其V3及邻区～ 30 0个核酸序列进行测定及分析。结果表明 ,19份样品中 11份为E亚型 ,5份为B亚型 ,1份为A亚型 ,1份为C亚型。上述资料表明 ,到目前为止流行于福建省的HIV - 1毒株的特点为以E、B亚型为主 ,多种亚型并存。两者型内以及与国际标准亚型序列的基因离散率均比较大。与其他省份的比较表明我省的毒株的传入与其他省份没有相关更多还原

【Abstract】 The fragments of the human immunodeficiency virus type 1(HIV-1) env gene was amplified by nest PCR from peripheral blood mononuclearcells (PBMCs) separated from blood of persons infected with HIV-1 and AIDS patient in Fujian province.The fragments were sequenced in their V3 regions and its flanks.The analysis results showed that the prevailing epidemic subtype of HIV-1 strains major is B and E in Fujian province.In comparison with the sequences between innersutypes,the consensus sequences of international subtypes and of subtypes in other provinces in China, the status of recent epidemic HIV-1 in Fujian province was realized.更多还原