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浙江省儿童隐孢子虫感染的诊断及分析
THE DIAGNOSIS AND ANALYSIS OF CRYPTOSPORIDIUM INFECTION IN CHILDERN OF ZHEJIANG PROVINCE
【摘要】 目的 对浙江省腹泻儿童进行隐孢子虫的病原学诊断及感染情况分析。方法 从浙江大学附属儿童医院收集腹泻儿童粪便548 份,每份样本先用改良抗酸染色法检查卵囊,发现阳性或可疑标本,再用免疫荧光染色法进一步确诊。结果 两种染色方法结合诊断共发现57 例阳性病人,隐孢子虫感染率为10-4% ,男女之间感染率无显著性差异(χ2 = 0-007,P> 0-05),其中1 岁以下婴儿感染率为5-7 % ,1 ~10 岁儿童感染率为17-5% ,两者之间存在显著性差异(χ2 = 19-69 ,P< 0-05)。结论 改良抗酸染色法与免疫荧光染色法结合诊断能达到隐孢子虫确诊目的,由感染情况分析可得浙江省腹泻儿童中有较高的隐孢子虫感染率,其中1 岁以下儿童感染率较1 岁以上儿童明显降低,可能与1 岁以下婴儿多母乳喂养有关。
【Abstract】 Aim To etiological diagnose and analyze of Cryptosporidium infection of diarrhea children in zhejiang province Method 548 fecal specimens were collected from diarrhea children in affiliated children hospital of Zhejiang College Each fecal specimen was smeared and screened by modified acid-fast staining method for oocyst;the positive and suspected specimens were identified by immunofluorescent staining method Result As a result,57 cases of infection were found by modified acid-fast staining and immunofluorescent staining method with a positive rate of 10 4%(57/548) Among the positive specimens 19 cases were under the age of l year,the positive rate was 5 7%(19/332),and 38 cases were between the age 2 and 10 with a positive rate of 17 5%(38/216) Conclusion The best result could be obtained when modified acid-fast staining method was first used and followed by immunofluorescent staining method The study showed that there were high Cryptosporidium infective rate in diarrhea children of Zhejiang province,but the positive rate under the age of 1 year was much lower than older children,this may be due to breast feeding
- 【文献出处】 中国人兽共患病杂志 ,CHINESE JOURNAL OF ZOONOSES , 编辑部邮箱 ,2000年01期
- 【分类号】R531.5
- 【被引频次】57
- 【下载频次】127