【摘要】 目的　探讨人类非小细胞肺癌组织中p16基因缺失及突变情况及其与临床病理的关系。 方法　应用控制模板DNA量的银染PCR-SSCP技术检测40例非小细胞肺癌手术切除标本中p16基因第2外显子。结果　40例中有13例发生纯合缺失，3例发生突变，缺失及突变率为40%。其缺失及突变率与肺癌的临床病理分期有密切关系（Ｐ<0.05）。 结论　 p16基因的缺失及突变可能在非小细胞肺癌的发生、发展及转移中起重要作用。更多还原

【Abstract】 ：Objective To study the relationship between the deletion and mutation of p16 gene and clinicopatho -logic manifestations of human non-small cell lung cancer(NSCLC). Methods Silver staining PCR-SSCP method of control amount of template DNA was used to detect exon 2 of p16 gene in human NSCLC tissue specimens. Results The deletion of p16 gene was identified in 13 out of 40 specimens. Of the 40 specimens, 3 showed a variant band indicative of the mutation. There was significant correlation between the frequency of the deletion and mutation(40%) and clinicopathologic stage(Ｐ<0.05). Conclusion The deletion and mutation of p16 gene may play an important role in carcinogenesis, development and metastasis of human NSCLC.更多还原