【摘要】 利用反转录 ( RT)和 PCR技术完成了 5株近期 ( 1 997～ 1 998年 )猪瘟流行毒和 1株猪瘟 C-株弱毒疫苗毒 (兰州 ) Erns(或称 E0 )基因的核酸序列测定。通过序列分析发现 ,这 5株流行毒与 C-株疫苗毒的核酸序列同源性为 83%～ 84%;推导的氨基酸序列同源性为 88%～ 91 %,我国 50～ 60年代流行的中国石门株 ( SM)的核酸序列同源性为 85%;氨基酸序列同源性为 89%～ 91 %。而 5株流行毒间的核酸序列同源性为 91 %～ 98%;氨基酸序列同源性为 94%～ 98%。更多还原

【Abstract】 The E rns gene of five prevalent virulent strains and one C strain of Hog Cholera Virus were amplified by reverse transcription(RT).The amplified fragments were cloned and sequenced.Comparison and analysis were made on the nucleotide sequence and the amino acid sequence of five prevalent virulent strains of HCV was deduced.The nucleotide homology was from 91% to 98%;the homology of amino acid was from 94% to 98%.The nucleotide homology of C srtain virus from cells cultured and five prevalent virulent strains of HCV was 83% to 84%.The amino acid homology was from 89% to 91%.更多还原