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棉花DNA导入苎麻引起变异的研究
Studies on Variation of Introducing Cotton DNA into Ramie
【摘要】 80年代以来,外源DNA直接导入植物的分子育种技术在国内得到迅速发展。就该技术本身而言,供、受体范围不断扩大,已达40余种〔1〕,但主要集中在禾本科和锦葵科的一年生大花作物;导入方法也在不断创新和改进,仍以周光宇创导的花粉管通道法为主,但对于多年生无性繁殖的小花作?..
【Abstract】 A new method of soaking superdried embryo was created for introducing exogenous cotton Xiangmian 12 DNA into ramie Xiangzhu 3 in this study. Plants with obvious variations on morphological characters were obtained in D 1 and D 2 generation and the results of RAPD assay indicated that cotton DNA had been integrated into ramie genome.In addition, the paper had preliminarily discussed the mechanism of soaking superdried embryo and the genetic stability of character of transformed offsprings.
【基金】 湖南省科委、计委重点资助
- 【文献出处】 中国农业科学 ,Scientia Agricultura Sinica , 编辑部邮箱 ,2000年01期
- 【分类号】S563.1
- 【被引频次】31
- 【下载频次】104