【摘要】 目的 :研究抗小鼠胸腺基质细胞 (MTSC)Pf18 3单抗识别分子的表达特性。方法 :采用流式细胞 (FACS)和激光扫描共焦显微镜 (CLSM)分析TSC单抗Pf18 3染色特征。结果 :FACS分析发现Pf18 3单抗识别分子可表达于胸腺、胎肝和骨髓等不同来源的基质细胞系和腹腔巨噬细胞表面。新鲜分离的胸腺细胞Pf18 3识别分子表达阴性 ,但ConA活化可诱导并上调其表达 ,随活化时间延长表达增高 ,且主要表达于CD4+CD8+和CD4+CD8-两细胞亚群。CLSM分析可见 :荧光沿胸腺细胞边缘呈环状分布 ,活化后荧光明显增厚向胞浆延伸 ,胞核不着色。结论 :Pf18 3单抗识别分子共表达于胸腺基质细胞及活化的胸腺细胞 ,并与CD4+CD8+和CD4+CD8-亚群胸腺细胞活化密切相关更多还原

【Abstract】 Objective: To identify the expression of the molecule recognized by Pf18 3 mAb (Pf18 3 molecule) on various cells Methods: The expression of Pf18 3 molecule was assayed by flow cytometry and confocal laser scanning microscope Results: The molecule recognized by Pf18 3 mAb expressed on TSC and other stromal cells Whereas, fresh thymocytes were Pf18 3 negative Interestingly, the expression of Pf18 3 molecule was gradually up regulated on thymocytes after activation by ConA This molecule mainly expressed on CD4 +CD8 + and CD4 +CD8 - cells Under confocal laser scanning microscope, the staining of fluorescence showed as ring around the cell,it changed gradually stronger and thicker with activation Conclusion: This study indicated that the Pf18 3 molecule was co expressive molecule of MTSC and activated thymocytes,it was concerned closely about the activation of CD4 +CD8 + and CD4 +CD8 - cells更多还原