【摘要】 目的 :观察外源性IL 4对K5 6 2增殖及死亡的影响。方法 :用逆转录病毒载体将IL 4cDNA导入K5 6 2细胞 ,检测其增殖反应 ,用流式细胞仪及DNA片段凝胶电泳检测细胞增殖周期及凋亡情况 ,用台盼蓝排除法计数死亡细胞。结果 :高表达IL 4的K5 6 2细胞增殖反应明显低于低表达株、亲代K5 6 2株 ;前者培养过程中死亡细胞百分率明显升高且可被IL 4单克隆抗体阻断 ,但其自发凋亡细胞数及增殖周期均无明显改变。结论 :外源性IL 4基因表达产物可诱导K5 6 2细胞死亡 ,但并非凋亡机制介导更多还原

【Abstract】 Objective: To observe the effect of exogenous IL 4 gene expression on cell death of K562 cells.Methods: IL 4 gene was transduced into K562 cell via retroviral vector mediated gene transfer method.Trypan blue stain,gel electrophoresis and flow cytometry were employed to determine cell death of K562 cell. Results:Proliferation of high IL 4 producing K562 clones was significantly lower than that of low IL 4 producing clones,parental K562 cells and K562 cells co cultured with recombinant IL 4.Cell death of high IL 4 producing K562 cells was enhanced dramatically and this effect could be blocked by IL 4 McAb,but its cell cycle no ever changed.There was no apoptosis determined.Conclusion: IL 4 expressed by IL 4 gene modified K562 cells could induce cell death of K562 cell by means of necrosis,which is benefitial for growth inhibition of leukemic cell.更多还原