【摘要】 对栀子 (Gardeniajasminoides)不同部位外植体进行无菌苗的诱导培养 ,诱导率表现为带腋芽的茎段 >顶芽 ,而叶片则不能被诱导发芽 .在继代过程中培养基MS +BA2 +NAA0 .5的继代效果好于培养基MS +KT0 .0 2 +NAA1.5.生根培养基 12 MS +IBA0 .5+NAA0 .1和 12 SH +IBA0 .5+NAA0 .1均有良好的生根效果 .移栽时 ,基质温度掌握在 18～ 2 0℃能够保证试管苗有良好的长势 .更多还原

【Abstract】 The different parts of explant of Gardenia jasminoides were made induced culture. Inductive rate presents stem with auxiliary bud>terminal bud, but leaves can’t be budded off. In the process of subculture, the subculture effect of culture medium MS+BA 2+NAA 0.5 is better than that of culture medium MS+KT 0.02 +NAA 0.5 . Culture medium of root initiation 12MS+IBA 0.5 +NAA 0.1 and 12SH+IBA 0.5 +NAA 0.1 have better effect. Tube seedlings have good growth under the condition of transplanting. The temperature of culture medium with 18～20?℃ during the process.更多还原