【摘要】 目的：观察用白细胞介素 ２（ｉｎｔｅｒｌｅｕｋｉｎ－２，ＩＬ－２）基因修饰小鼠骨髓来源的树突状细胞（ｄｅｎｄｒｉｃ ｃｅｌｌｓ， ＤＣ）后，ＤＣ表达细胞因子和趋化因子的变化及ＤＣ体内免疫后，小鼠体内主要脏器中ＩＬ－２表达情况。探讨其对抗原提呈微环境的改善及其可能的机制。方法：用ＥＩＪＳＡ方法检测ＤＣ培养上清和小鼠血清及脏器中ＩＬ－２的含量，用ＲＴ－ＰＣＲ方法检测ＤＣ中ＩＬ－２，ＩＦＮ－γ，ＩＬ－１２，ＧＭ－ＣＳＦ，ＩＬ－４，ＩＬ－１０，ＴＮＦ－α，ＩＬ－１－β，ＭＩＰ－１β，ＭＣＰ－１，ＲＡＮＴＥＳ和ＩＰ１０等的表达，用ＦＡＣＳ分析ＤＣ表面ＩＬ－２受体的变化。结果：经ＩＬ－２基因修饰后，ＤＣ除了分泌高水平的ＩＬ－２外，尚能分泌一定水平的ＩＦＮ－γ和ＩＬ－１２，其表面表达的ＩＬ－２Ｒα密度增加。ＲＴ－ＰＣＲ分析表明其表达多种细胞因子和趋化因子，用ＩＬ－２基因修饰的ＤＣ免疫小鼠后，血清和脏器中能检测到高水平的ＩＬ－２。结论：ＩＬ－２基因修饰ＤＣ，能促进ＤＣ表达ＩＬ－２受体，并以自分泌形式分泌高水平的ＩＬＺ，优化其抗原提呈的微环境，提示可增强ＤＣ的抗原提呈功能。更多还原

【Abstract】 Objective: To investigate the effect of IL-2 gene modification on expression of cytokines and chemokines in dendritic cells (DCs) and IL-2 expression in mouse organs after in the DC Immunization and to determine the improvement of microenvi- ronment of antigen presentation and its potential mechanism. Methods: The IL-2 levels in DC culture, mouse serum and organs after IL-2 gene-modified DC(DC-IL-2) injection in vivo were examined by ELISA. The mRNA expressions of cytokine and chemokines (IL-2, IDN-γ, IL-12, GM-CSF, IL-4, IL-10, TNF-α, IL－1β, MIP-1β, MCP-1, RANTES and IP10) in DC-IL-2 were analyzed by RT-PCR. The expression of IL-2Rα on sulfate of DC-IL-2 was analyzed by FACS. Results: After IL-2 gene modification, DCs could secret high level of IL-2, IFN-γ and IL-12. The expression of IL-2Rα on the surface of DC-IL-2 was in- creased. mRNAs of various cytokines and chemokines were detected. The levels of IL-2 increased immediately in mouse scrum, lung, spleen and liver after DC-IL-2 injection in vivo. Conclusion: The IL-2 gene modification could enhance the biologic activ- ity of the DCs and the microenvironment of antigen presentation was optimized.更多还原