【摘要】 目的　评价肿瘤细胞经药物处理后的生存情况 ,探讨将该方法用于卵巢癌药敏试验的可行性。方法　采用生物荧光法检测三磷酸腺苷 (ATP) ,对小鼠纤维瘤细胞株L92 9、人卵巢癌组织和腹水细胞进行抗癌药物处理后的细胞活性测定。结果　该法能测出不同浓度药物对细胞作用的剂量—效应关系 ,以及不同个体对不同浓度药物反应的差异 ,实验变异系数CV值为 1 2～ 15 8%。结论　ATP生物荧光法有稳定、重复性好、特别敏感的特点 ,用于卵巢癌体外药敏试验可行。更多还原

【Abstract】 Adanosine triphosphate(ATP) is the basic energy source for living cells.It rapidly disappears when cells die.So measuring the ATP levels may estimate the viability of cells exposed to drugs.Dose-response curve can be determined in mouse fibroma cell line:L929 treated with chemotherapeutic agents by the ATP-bioluminescence assay and The different in vitro response of 6 ovarian carcinoma (5 from fresh tumor tissues,1 from ascites)treated with etopside,cis-plating,5-fluorouracil and adriamycin can be investigated.The coefficient of variation for triplicate assays ranges from 1.2 to 15.8%.It means high reproducibility of the assay.This presentation also reported that single cell suspension(include <30 cells clusters) can be separated from tissue fragments by means of the enzyme cocktail(collagenase,Dnase,pronase).The viable cells were over 90%.0.5% Argarose/McCoy underlayer can prevent the growth of anchorage-dependent nonmalignant cells.This study demonstrates that ATP bioluminescence assay is a sensitive,efficient method for tumor chemosensitivity test.Correlation of in vitro drug sensitivity and in vivo patient response worth further studying.更多还原