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用重组 rK39抗原试纸条快速诊断内脏利什曼病(英文)

RAPID SCREENING WITH A RECOMBINANT ANTIGEN (rK39) FOR DIAGNOSIS OF VISCERAL LEISHMANIASIS USING DIPSTICK *

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【作者】 瞿靖琦管立人依马木苏里单左新平柴君杰陈生邦Shinichiro KawazuKenKatakuraYoshigeruMatsumotoS.G.ReedK.-P.Chang

【Author】 QU Jing qi 1, GUAN Li ren 1, Imamu Shulidan 2, ZUO Xin ping 2, CHAI Jun jie 2, CHEN Sheng bang 3, Shinichiro Kawazu 4, Ken Katakura 5, Yoshigeru Matsumoto 6, S. G. Reed 7 K. P. Chang 8 [WT5”HX](1 Institute of Parasit Diseases, Chin

【机构】 中国预防医学科学院寄生虫病研究所!上海200025新疆维吾尔自治区地方病防治研究所!乌鲁木齐830002甘肃地方病防治研究所!兰

【摘要】 [目的 ]评价以恰氏利氏曼原虫类 kinesin基因中编码 39个氨基酸的基因片段 (r K39)为重组抗原 ,用于血清学诊断内脏利什曼病的价值。 [方法 ]在新疆喀什地区对 13例经脾检和骨髓穿刺阳性的内脏利什曼病患者 ,取一滴病人全血或血清滴在 r K39抗原试纸条底部的吸收垫上 ,血清中蛋白随缓冲液向试纸条上部移动 ,其中相应特异抗体可与 r K39抗原带结合 ,而产生阳性条带。同时 ,本文亦用相同阳性血清作了关于 r K 39抗原的 Western印迹分析对照。 [结果 ]EL ISA分析显示病人血清抗体滴度在 10 - 2~ 10 - 4 ,与所见到的 r K39试纸条上的反应强度符合。Western印迹分析亦显示阳性血清可识别 r K39蛋白条带。[结论 ]与传统诊断内脏利什曼病方法相比较 ,r K39试纸条更快速 ,特异 ,灵敏和低损伤性 ,可用于低发病率流行区的内脏利什曼病的诊断和筛选

【Abstract】 Objective] To evaluate the diagnostic value of the recombinant antigen of 39 amino acid repeats encoded by a kinesin like gene of Leishmania changasi (rK39) in serodiagnosis of visceral leishmaniasis (VL). [Methods] In Kashi, Xinjiang, 13 VL patients with splenomegaly and bone marrow aspirate culture positive were subjected to dipstick assay. A drop of whole blood or serum from patient was placed at the absorbing pad at the bottom of the dipstick. Flooding of the bottom protein with buffer allows serum proteins to migrate upwards, producing the positive band and Western blot analysis of rK39 subsequently performed with the sera collected. [Results] The end point titers of anti rK39 antibodies of these sera were determined by ELISA and found to fall within the range of 10 -2 to 10 -4 , which were consistent with the intensity of their reaction with rK39 in dipstick assay. The positive sera could also recognize the specific rK39 band as analyzed by Western blot analysis. [Conclusion] The rK39 dipstick assay is more rapid, specific, sensitive and less invasive than the conventional methods of diagnosis for VL in the areas of low endemicity.

【基金】 日本文部省资助课题
  • 【文献出处】 中国寄生虫学与寄生虫病杂志 ,CHINESE JOURNAL OF PARASITOLOGY AND PARASITIC DISEASES , 编辑部邮箱 ,2000年03期
  • 【分类号】R446.61
  • 【被引频次】6
  • 【下载频次】44
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