【摘要】 [目的 ]探索弓形虫感染早期诊断的简便方法。 [方法 ]用SPA ELISA检测轻度、中度和重度感染弓形虫鼠尿中弓形虫循环抗原 (TCA)。 [结果 ]轻度、中度和重度感染弓形虫 3组鼠尿中均检出TCA ,上述 3组分别于弓形虫感染后d6 、d3和d4显示阳性斑点。 [结论 ]SPA ELISA能检出弓形虫感染鼠尿中的弓形虫循环抗原 ,可用于弓形虫感染的早期诊断。更多还原

【Abstract】 Objective] To explore a simple and convenient immunoassay for early diagnosis of Toxoplasma infection.[Methods] Urine samples collected from three groups of mice infected with different doses of tachyzoites were detected for Toxoplasma circulating antigen (TCA) by dot ELISA using HRP SPA as a second antibody ( SPA ELISA).[Results] Toxoplasma circulating antigens were detected in all three groups of infected mice in contrast with the normal control group. Toxoplasma circulating antigen was detected on d 6 and d 3 after infection in mice of light and moderate infection groups, respectively.[Conclusion]SPA ELISA is a simple and convenient method for early immunodiagnosis of recent Toxoplasma infection.更多还原