【摘要】 为探讨化疗对日本血吸虫 31/ 32 k Da抗体的影响 ,应用纯化日本血吸虫 31/ 32 k Da抗原 (Sj31/ 32 )和可溶性虫卵抗原 (SEA)对 94例经吡喹酮治疗后不同时期血吸虫病患者血清抗体进行检测。结果治疗后 3和 6个月血清31/ 32 k Da Ig G水平分别为 1.2 6± 0 .15和 0 .6 5± 0 .14(治疗前为 2 .0 3± 0 .2 0 ,P<0 .0 1) ,而 SEA Ig G水平则分别为 2 .87± 0 .19和 2 .30± 0 .2 0 (治疗前为 3.0 1± 0 .17,P>0 .0 5 )。各年龄组治疗前后血吸虫抗体水平均随治疗进展而降低 ,其中儿童和青少年 (3～ 2 0岁年龄组 ) Sj31/ 32 Ig G水平下降最快 ,治疗后 6个月时几乎接近正常值。治疗前Sj31/ 32 Ig G与 SEA Ig G水平之间高度相关 (r=0 .90 ) ,治疗后 3和 6个月两者之间的相关程度降低 (r分别为 0 .30和 0 .40 )。结果表明 ,吡喹酮治疗后患者血清中 Sj31/ 32 Ig G下降较快 ,Sj31/ 32 Ig G似可作为一种短期抗体用于疗效考核更多还原

【Abstract】 To probe the effects of chemotherapy on Schistosoma japonicum 31/32 kDa antibody, purified S. japonicum 31/32 kDa antigen(Sj31/32) and soluble egg antigen(SEA) were used to quantitatively detect the antibodies in sera from 94 patients infected S. japonicum after praziquantel treatment for 3 and 6 months. The results showed that the levels of Sj31/32 IgG were 1.26 ±0.15 and 0.65±0.14(2.03±0.20 before treatment, P <0.01), while the levels of SEA IgG were 2.87±0.19 and 2.30±0.20 (3.01±0.17 before treatment, P >0.05) respectively after treatment for 3 and 6 months. The levels of Sj31/32 IgG and SEA IgG declined with the process of treatment in all age groups. The level of Sj31/32 IgG dropped the most quickly in the age of 3~20. It would be almost reduced to the normal value after treatment for 6 months. There was a significant correlation between the level of Sj31/32 IgG and that of SEA IgG before treatment(r=0.90), while it were decreased after treatmeat for 3 and 6 months(r=0.30,0.40 respectively). The results suggested that the level of Sj31/32IgG declined more quickly after treatment than that of SEA IgG. Sj31/32IgG might be a short duration antibody which could be used as evaluation of therapeutic efficacy.更多还原