【摘要】 为建立抗青蒿琥酯恶性疟原虫虫株 ,将采自恶性疟病人血 ,用 Trager法进行体外连续培养 ,待其正常生长后 ,在培养基中加不同浓度的青蒿琥酯进行培育 ,并在培育前及用药后不同时间用 Rieckmann体外微量法测定青蒿琥酯半数抑制量 (ID5 0 )。用药前及用药后 6 8d、12 9d及停药后 6 7d的 ID5 0 分别为 9.6、30 .6、85 .1及 5 2 .9nm ol/L。结果表明可用人工方法建立高度抗青蒿琥酯恶性疟原虫虫株 ,停药后抗性程度有所下降更多还原

【Abstract】 To culture Plasmodium falciparum strain resisting artesunate. The isolated strain collected from patient was cultured in vitro by Trager method. After isolated strain grows normally, different concentrations of artesunate were added into the culturing plates to culture. ID 50 of artesunate was assayed by Rieckmann’s in vitro micro technique at different time. Before and after adding drugs for 68 d, 129 d and stopping drug for 67 d, ID 50 of artesunate was 9.6, 30.6, 85.1 and 52.9 nmol/L respectively. The results suggested that P. falciparum strain high resisting artesunate can be cultured by manual work.更多还原