【摘要】 弓形虫棒状体蛋白 1(ROP1)是与虫体侵入相关的重要分子。将含编码 ROP1蛋白基因的真核表达重组质粒 pc DNA3转染 Hep G- 2细胞 ,为进一步研究做准备。用脂质体介导的真核细胞转染法 ,G418筛选阳性克隆 ,SDS-PAGE及 Western- blot分析鉴定表达产物。结果显示 ,在所筛选的克隆化生长的细胞裂解液样品电泳图谱中 ,有一大小约 35～ 40 k Da的特异电泳条带 ,该条带可被弓形虫免疫兔血清识别。本研究建立了体外稳定表达 ROP1的细胞系 ,证实了表达产物的免疫原性更多还原

【Abstract】 Rhoptry protein 1 (ROP1) from Toxoplasma gondii was an important molecular related with the host cell penetrating. To prepare for further study, the eukaryotic expression recombinant pcDNA3 ROP1 was introduced into mammalian cells, HepG 2, using lipsome mediated transfection. The transfected cell clones were selected by G418 culturing,and the cells and their cultured fluid were tested by SDS PAGE and Western blot. The results showed that figure of SDS PAGE and Western blot revealed that a band about 35-40 kDa in the sample of pcROP1/HepG2 cell lysis could be recognized by rabbit antiserum of T. gondii . pcROP1/HEPG 2, and a stable expression system in vitro has been established and the antigenicity of the recombinant protein has been confirmed.更多还原