【摘要】 为了解抗青蒿琥酯恶性疟原虫对本芴醇、蒿甲醚、双氢青蒿素是否存在交叉抗性 ,本芴醇与青蒿琥酯伍用是否有增效作用 ,运用 Rieckmann体外微量法测定。结果上述 3种药物对青蒿琥酯敏感株恶性疟原虫的 ID5 0 分别为 72 .3、14.6及 13.4nm ol/ L ;对抗性株的 ID5 0 依次为 6 6 .8、80 .5及 72 .6 nmol/ L。蒿甲醚、双氢青蒿琥酯对抗性株的 ID5 0 分别较敏感株高出 4.5及 4.4倍 ,本芴醇对敏感株的 ID5 0 与抗性株相似。在本芴醇与青蒿琥酯伍用中 ,两者对抗性株的 ID5 0 分别为 44 .8及 39.6 nmol/ L ,为单用组的 1/ 1.5 4(44 .7/ 6 6 .8)和 1/ 2 .2 (39.6 / 85 .1)。结果提示抗青蒿琥酯恶性疟原虫对本芴醇无交叉抗性 ,青蒿琥酯与本芴醇伍用在体外测定中具有一定增效作用 ;抗青蒿琥酯恶性疟原虫对蒿甲醚、双氢青蒿素有明显的交叉抗性更多还原

【Abstract】 In order to compare sensitivity of artesunate sensitive and resistant P. falciparum isolates to benflumetolum, artemether and dihydroarteannuin, Reickmanns in vitro micro technique was used. ID 50 of benflumetolum, artemether and dihydroarteannuin were 72.3, 14.6 and 13.4 nmol/L to the sensitive isolates respectively; ID 50 of the 3 drugs as above were 66.8, 80.5 and 72.6 nmol/L to the resistant isolates respectively. On comparison of the sensitive and the resistant, ID 50 of benflumetolum has not changs, artemether and dihydroarteannuin ID 50 are 4.5 and 4.4 times higher than the former. In Benflumetolum combined with artesunate, ID 50 of the 2 drugs were 44.8 and 39.6 nmol/L respectively, the ID 50 is 1/1.5 (44.7/66.8) and 1/2.2 (39.6/85.1) of the single drug used groups. The results suggested that the resistant isolates of P. falciparum have sign of crossing resistance to artemether and dihydroarteannuin, but have no cross resistance to benflumetolum. Benflumetolum combined with artesunate has an increasing effectiveness to the resistant isolates in vitro test.更多还原