【摘要】 目的　探讨慢性粒细胞白血病 (chronicmyelocyticleukemia,CML)细胞黏附分子L selectin(CD62L)、Mac 1(CD11b)、LFA 1(CD11a)的表达与CML的发生、疗效的关系。方法　采用三色流式细胞仪检测 34例CML骨髓CD34+CD38-～ +细胞表面LFA 1、Mac 1、L selectin的表达。结果　初治的CMLCD34+CD38-～ +细胞黏附分子L selectin、LFA 1明显低于正常造血细胞的表达。L selectin的表达与Ph′阳性细胞数呈负相关 (r=- 0 .6 8)。 8例CML经干扰素治疗后 ,其L selectin、LFA 1表达可恢复到正常水平。 2例异体骨髓移植后的CML患者 ,其L selectin、Mac 1、LFA 1的表达均正常。结论　CMLCD34阳性细胞黏附分子L selectin、LFA 1的低表达反映了CML细胞的特点之一 ,α 干扰素及异体骨髓移植可恢复L selectin、LFA 1、Mac 1的表达更多还原

【Abstract】 Objective To study the expression of L selectin, Mac 1, LFA 1 on CML progenitor cells in relation to CML progression and therapeutic effect.Methods The expression of adhesion molecules (LFA 1, Mac 1, L selectin) on bone marrow CD34 + cells from 34 CML patients were analyzed by three color flow cytometry.Results The mean percentage of expression of L selectin, and LFA 1 on CD34 +CD38 -～+ cells from untreated CML patients was significantly lower than that from normal controls. Among 8 CML patients treated with IFN α, the expression of L selectin and LFA 1 on CD34 + CD38 - cell (37.6±5.3%, 42.1±13.1%) was comparable to that from normal controls (38.2±9.4%, 48.2±12.2%). L selectin expression in CD34 +CD38 - cells from CML patients was inversely correlated with the percentage of Ph′(+) cells. In 2 CML patients treated with allo bone marrow transplantation, the expression rate of L selectin, IFA 1 and Mac 1 on CD38 +CD38 - cells was comparable to that from normal controls.Conclusion The data suggest that decreased expression of L selectin and LFA 1 in CML CD34 + cells reflects one of the features of malignant CML progenitors. IFN α and allo BMT restore the expression of Mac 1, L selectin and LFA 1 to normal on CML CD34 + cells.更多还原