【摘要】 目的　探讨人大肠癌培养细胞p5 3存在状态与化疗敏感性的相关性。方法　应用p5 3机能诊断法 ,确认 6株人大肠癌培养细胞p5 3的存在状态 ,以MTT方法比较了p5 3野生型和突变型两组肠癌细胞对化疗敏感性的差异 ,用流式细胞仪做细胞周期的进一步分析。结果　p5 3野生型肠癌细胞较p5 3突变型细胞的化疗敏感性高 5～ 10倍 ,并且在化疗药阿霉素诱导下可选择地发生细胞凋亡和G1阻滞。结论　人体大肠癌培养细胞p5 3存在状态直接与其化疗敏感性有关。更多还原

【Abstract】 Objective To study the functional status of tumor suppressor gene p53 in relation to chemotherapeutic sensitivity in colon cancer cell lines.Methods With the use of functional assay (FASAY) of p53, the existing p53 stuatus (wt, mt) of 6 colon cancer cell lines was determined. The difference in chemotherapeutic sensitivity of colon cancer cells with wild type p53 was compared with those with mutated type p53 by MTT method, followed by cytometic analysis of cell cycle.Results The chemotherapeutic sensitivity of Lovo and LS 174T cancer cell lines with wild type p53 was 5～10 times higher than that of 4 other cell lines with mutated p53. Selective apoptosis and cell cycle arrest in G 1 phase occurred when treated with adriamycin.Conclusion The functional status of p53 in human colon cancer cell lines directly affects their sensitivity to chemotherapeutic agents.更多还原