【摘要】 目的　研究致癌物代谢酶细胞色素P45 0 2E1基因 (CYP2E1)多型性与肺癌风险的关系。方法　以PCR RFLP方法 ,分析 92例肺癌患者和 137例正常对照者RsaI识别的CYP2E1基因型。结果　c1/c1基因型频率在肺癌病例组为 72 8% ,显著 (P <0 0 1)高于对照组的 5 4 7%。多因素分析表明 ,携带c1/c1的个体发生肺癌的危险性比携带c1/c2和c2 /c2的个体高 2 5倍 (OR 2 5 ,95 %CI 1 8～ 3 8)。分层分析发现 ,c1/c1基因型主要增加肺鳞状细胞癌的危险性 (OR 2 6 ,95 %CI 2 3～ 5 8)。重要的是 ,研究发现CYP2E1c1/c1基因型与吸烟有协同作用。c1/c1基因型或吸烟单因素作用的OR分别为 3 9和 4 1,而二者联合作用的OR为 7 9;当吸烟量 <2 0包 年时 ,c1/c2和c2 /c2基因型的OR为 2 4,而c1/c1基因型的OR为 7 6 ;当吸烟量≥ 2 0包 年时 ,前者的OR为 5 5 ,而后者的OR增加到8 7。结论　CYP2E1c1/c1基因型是中国人肺癌的遗传易患性因素 ,此种基因型与吸烟有协同作用。更多还原

【Abstract】 Objective To investigate the genetic polymorphism of cytochrome P4502E1 ( CYP2E1 ) as a susceptibility factor for lung cancer in Chinese population. Methods The genotype frequencies of RsaI restriction fragment length polymorphisms were analyzed in 92 lung cancer cases and 137 frequency matched normal controls. Results The CYP2E1 c1/c1 genotype was found in 72.8% of lung cancer cases, which was significantly higher ( P <0.01) than that in controls (54.7%). By multivariate analysis, this genotype was found to be associated with a 2.5 fold increased risk of lung cancer (adjusted OR 2.5, 95% CI 1.8 3.8). Stratified analysis suggested an interaction between CYP2E1 c1/c1 genotype and cigarette smoking. The odds ratio (OR) for the CYP2E1 c1/c1 genotype, cigarette smoking and both factors combined was 3.9 (95% CI 1.1 15.2), 4.1 (95% CI 1.1 15.9) and 7.9 (95% CI 2.4 29.4), respectively. The interaction between CYP2E1 c1/c1 genotype and pack years of cigarette smoking followed the same pattern, with a markedly increased risk in the heaviest smokers. Conclusion The CYP2E1 c1/c1 genotype is a susceptibility factor for development of lung cancer in Chinese and there is an apparent gene environment interaction between the susceptible genotype and cigarette smoking.更多还原