【摘要】 目的　调查我国戊型肝炎病毒基因型分布。方法　应用聚合酶链反应法和直接测序法对从我国 14个城市分离的 45株戊型肝炎病毒开放读码框架 2 (ORF2 )的部分核苷酸序列 (nt6 46 1 6 86 0及nt5 994 6 2 94)进行分析。结果　 45株散发性戊型肝炎病毒 (HEV)中 ,41株 (91% )与缅甸株 (B)属同一个基因型 ,与典型中国株HEV核苷酸序列的同源性均在 98%以上。其余 4株变异较大 ,其序列与HEV缅甸株 (B) /中国株、墨西哥株 (M)、美国株 /猪 (US/Swine)的同源性仅分别为 77%～ 80 %、74%～76 %和 74%～ 77% ;氨基酸序列的同源性分别为 91%～ 95 %、89%～ 92 %和 93%～ 97%。基因进化树分析表明 ,该 4株HEV变异株可能为一个新基因型 ,它不同于HEV(B)、HEV(M)和HEVUS/Swine ,按其核苷酸序列的差异可划分为 2个亚型。结论　我国绝大多数戊型肝炎患者为典型中国株HEV感染 ,仅少数患者可能为新的基因型HEV感染。更多还原

【Abstract】 Objective To investigate the genotypes of hepatitis E viruses isolated in China. Methods The partial genome of open reading frame 2 (nt6461-6860, nt5994-6294) of 45 HEV isolated from patients in 14 cities of China was amplified and sequenced using polymerase chain reaction and direct sequencing. Results 41 of 45 isolates (91%) shared the same genotype with HEV Burma strain (B), with nucleotide identities higher than 98% with the representative HEV Chinese strain. Only 4 HEV isolates were significantly divergent from the 3 prototype strains of HEV, with nucleotide identities of 77%-80% with HEV Burmese/Chinese strain, 74%-76% with Mexico strain and 74%-77% with the newly discovered HEV US/Swine strain, respectively. Phylogenetic analysis suggested that these 4 isolates represented 2 different subtypes belonging to a novel genotype of HEV, which was significantly divergent from the prototype Mexican, Burmese and US/Swine isolates. Conclusion Among patients with hepatitis E in China, most are infected with the Chinese prototype HEV, and only a small part with the new genotype HEV.更多还原