【摘要】 目的　了解睾丸获取精子结合卵浆内单精子注射治疗无精子症的疗效。方法　采用睾丸精子卵浆内显微注射方法 ,对 30对无精子症不育症夫妇进行了辅助生育。在女方超排卵治疗获卵当天 ,通过睾丸活检取出曲细精管 ,从中分离精子或应用圆形精子细胞注射入卵浆辅助受精 ,形成胚胎后移植入宫腔。结果　阻塞性无精子症组 10例患者接受了 11个治疗周期 ,受精率 6 7.0 % (6 9/10 3) ,平均移植 3.8个胚胎 ,妊娠 2例 ;非阻塞性无精子症组 2 0例患者接受了 2 1个治疗周期 ,仅 12例患者 13个周期获得精子行睾丸精子卵浆内显微注射 ,受精率 5 9.0 % (72 / 12 2 ) ,11个周期有胚胎移植 ,平均移植 2 .8个胚胎 ,妊娠 3例。首对夫妇已于 1998年 6月孕足月分娩一正常男婴。结论　虽然用睾丸精子卵浆内显微注射的难度较高 ,但可用于辅助无精子症患者生育 ,其疗效及获得精子率仍有待进一步提高。更多还原

【Abstract】 Objective To investigate the effect of intracytoplasmic sperm injection (ICSI) on providing fertility for obstructive and non obstructive azoospermia men after testicular spermatozoa extraction (TESE). Methods TESE and ICSI were performed at the day of ovum pick up. Results TESE and ICSI were performed successfully for 11 cycles in obstructive azoospermia men; The fertilization rate (FR) and clinical pregnancy rate (PR) were 67.0%(69/103) and 18.2%(2/11), respectively. In non obstructive azoospermia group (21 cycles) including 3 cases of severe oligozoospermia, only in 13 cycles could sperm be found to perform ICSI, with the FR of 59.0%(72/112); 11 cycles had embryo transfer, and the average embryo number for transfer was 2.8. Three cases got clinical pregnancy. In 8 cycles, TESE were failed. The first case delivered a normal boy in June 1998. Conclusions Using testicular sperm for ICSI is relatively difficult, but it is a practical method to provide fertility for obstructive or non obstructive azoospermia men. Its efficacy, however, is needed to be improved.更多还原