【摘要】 目的　观察培养生长于羊膜的角膜缘干细胞移植治疗角膜缘碱烧伤的效果。方法　将兔角膜缘干细胞原代培养后接种于羊膜 ,对新西兰大白兔角膜缘碱烧伤动物模型行角膜缘干细胞羊膜移植术 ,并对治疗后的角膜进行临床及病理学检查。结果　体外培养的兔角膜缘干细胞可在羊膜上继续增殖、分化为密集的角膜上皮细胞层 ;角膜缘干细胞移植术后兔角膜缘轻度充血、角膜上皮完整、基质细胞浸润减轻、新生血管减少。组织病理学染色证实 :角膜缘及其周边上皮细胞为多层结构 ,角膜新生血管消失及基质中炎性细胞浸润减轻。结论　角膜缘干细胞羊膜移植术治疗角膜碱烧伤可恢复其角膜上皮结构的完整性 ,减少角膜新生血管的形成 ,维持角膜缘的细胞屏障功能 ,为进一步行角膜移植创造条件。更多还原

【Abstract】 Objective To study the treatment of rabbit limbal alkali burn by cultured limbal stem cells growing on amniotic membrane for allograft transplantation Methods After the primary culture of the rabbit corneal stem cells, they were cultured on amniotic membrane in DMEM/HamF12 medium for one week Corneal stem cells and amniotic membrane were tramsplanted on the limbal and scleral area of the rabbit model with alkali burn The corneal changes were observed by a slitlamp everyday, and the corneal pathological changes were examined Results Cultured rabbit corneal stem cells continued to proliferate, differentiate and form multiple cell layers on amniotic membrane After transplantation with cultured stem cells and amniotic membrane, the rabbit epithelium showed corneal phenotype and progressive decrease of vascularity and stromal infiltration in the limbal and peripheral zone Pathological examination verified that the limbal and peripheral corneal epithelium was composed of multilayer cells, the neo vascularization was reduced and stromal inflammatory cells were decreased Conclusions Allograft transplantation with cultured limbal stem cells can restore corneal epithelial cell composition, decrease neovascularization, maintain limbal cellular barrier function and provide better condition for keratoplasty later更多还原